A technique is described for embedding tissue culture cells that have been adsorbed or grown on Millipore filters. The acetone used during the embedding process rendered the filters transparent so that specific areas or cells could be chosen with the aid of the light microscope. Lymphoblastoid cells processed on the filters possessed well-defined plasma membranes and microvilli which were rarely present in cells from parallel cultures that were prepared by pelleting in the centrifuge. Fibroblast cells grown on filters retained their elongated appearance, in contrast to the rounded cells in pelleted preparations. Millipore filters were also used as a means of embedding virus pellets for sectioning. Preparations containing as few as 4 x 108 virus particles were suitable for study by the filter technique. Crude tissue-culture harvests of vaccinia virus and purified preparations of Rauscher murine leukemia and adeno-satellite viruses were successfully examined.
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机译:描述了一种用于将已经吸附或生长在Millipore过滤器上的组织培养细胞包埋的技术。嵌入过程中使用的丙酮使滤镜透明,以便可以借助光学显微镜选择特定的区域或细胞。在过滤器上处理过的淋巴母细胞具有清晰的质膜和微绒毛,在平行培养的细胞中很少存在,这些细胞是通过离心沉淀形成的。与颗粒状制剂中的圆形细胞相反,在过滤器上生长的成纤维细胞保留了其细长的外观。密理博过滤器还用作嵌入病毒颗粒进行切片的方法。含有少至4 x 108病毒颗粒的制剂适合通过过滤技术进行研究。成功检查了牛痘病毒的粗组织培养收成,劳氏鼠白血病和腺卫星病毒的纯化制剂。
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